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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Soja. |
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Data corrente: |
10/03/2006 |
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Data da última atualização: |
04/10/2007 |
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Autoria: |
ALBERTON, O.; KASCHUK, G.; HUNGRIA, M. |
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Título: |
Sampling effects on the assessment of genetic diversity of rhizobia associated with soybean and common bean. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Soil Biology and Biochemistry, Oxford, v. 38, p. 1298-1307, 2006. |
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Idioma: |
Inglês |
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Conteúdo: |
Biological nitrogen fixation plays a key role in agriculture sustainability, and assessment of rhizobial diversity contributes to worldwide knowledge of biodiversity of soil microorganisms, to the usefulness of rhizobial collections and to the establishment of long-term strategies aimed at increasing contributions of legume-fixed N to agriculture. Although in recent decades the use of molecular techniques has contributed greatly to enhancing knowledge of rhizobial diversity, concerns remain over simple issues such as the effects of sampling on estimates of diversity. In this study, rhizobia were isolated from nodules of plants grown under field conditions, in pots containing soil, or in Leonard jars receiving a 10-2 or a 10-4 serially-diluted soil inoculum, using one exotic (soybean, Glycine max) and one indigenous (common bean, Phaseolus vulgaris) legume species. The experiments were performed using an oxisol with a high population (105 cells g-1 soil) of both soybean rhizobia, composed of naturalized strains introduced in inoculants and of indigenous common-bean rhizobia. BOX-PCR was used to evaluate strain diversity, while RFLP-PCR of the ITS (internally transcribed spacer) region with five restriction enzymes aimed at discriminating rhizobial species. In both analyses the genetic diversity of common-bean rhizobia was greater than that of soybean. For the common bean, diversity was greatly enhanced at the 10-4 dilution, while for the soybean dilution decreased diversity. Qualitative differences were also observed, as the DNA profiles differed for each treatment in both host plants. Differences obtained can be attributed to dissimilarity in the history of the introduction of both the host plant and the rhizobia (exotic vs. indigenous), to host-plant specificity, rhizobial competitiveness, and population structure, including ease with which some types are released from microcolonies in soil. Therefore, sampling method should be considered both in the interpretation and comparison of the results obtained in different studies, and in the setting of the goals of any study, e.g. selection of competitive strains, or collection of a larger spectrum of rhizobia. Furthermore, effects of sampling should be investigated for each symbiosis. MenosBiological nitrogen fixation plays a key role in agriculture sustainability, and assessment of rhizobial diversity contributes to worldwide knowledge of biodiversity of soil microorganisms, to the usefulness of rhizobial collections and to the establishment of long-term strategies aimed at increasing contributions of legume-fixed N to agriculture. Although in recent decades the use of molecular techniques has contributed greatly to enhancing knowledge of rhizobial diversity, concerns remain over simple issues such as the effects of sampling on estimates of diversity. In this study, rhizobia were isolated from nodules of plants grown under field conditions, in pots containing soil, or in Leonard jars receiving a 10-2 or a 10-4 serially-diluted soil inoculum, using one exotic (soybean, Glycine max) and one indigenous (common bean, Phaseolus vulgaris) legume species. The experiments were performed using an oxisol with a high population (105 cells g-1 soil) of both soybean rhizobia, composed of naturalized strains introduced in inoculants and of indigenous common-bean rhizobia. BOX-PCR was used to evaluate strain diversity, while RFLP-PCR of the ITS (internally transcribed spacer) region with five restriction enzymes aimed at discriminating rhizobial species. In both analyses the genetic diversity of common-bean rhizobia was greater than that of soybean. For the common bean, diversity was greatly enhanced at the 10-4 dilution, while for the soybean dilution decreased diversity. ... Mostrar Tudo |
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Palavras-Chave: |
Diversidade genética; Rizóbio. |
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Thesagro: |
Rhizobium; Soja. |
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Thesaurus Nal: |
soybeans. |
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Categoria do assunto: |
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Marc: |
LEADER 02857naa a2200205 a 4500
001 1629207
005 2007-10-04
008 2006 bl --- 0-- u #d
100 1 $aALBERTON, O.
245 $aSampling effects on the assessment of genetic diversity of rhizobia associated with soybean and common bean.
260 $c2006
520 $aBiological nitrogen fixation plays a key role in agriculture sustainability, and assessment of rhizobial diversity contributes to worldwide knowledge of biodiversity of soil microorganisms, to the usefulness of rhizobial collections and to the establishment of long-term strategies aimed at increasing contributions of legume-fixed N to agriculture. Although in recent decades the use of molecular techniques has contributed greatly to enhancing knowledge of rhizobial diversity, concerns remain over simple issues such as the effects of sampling on estimates of diversity. In this study, rhizobia were isolated from nodules of plants grown under field conditions, in pots containing soil, or in Leonard jars receiving a 10-2 or a 10-4 serially-diluted soil inoculum, using one exotic (soybean, Glycine max) and one indigenous (common bean, Phaseolus vulgaris) legume species. The experiments were performed using an oxisol with a high population (105 cells g-1 soil) of both soybean rhizobia, composed of naturalized strains introduced in inoculants and of indigenous common-bean rhizobia. BOX-PCR was used to evaluate strain diversity, while RFLP-PCR of the ITS (internally transcribed spacer) region with five restriction enzymes aimed at discriminating rhizobial species. In both analyses the genetic diversity of common-bean rhizobia was greater than that of soybean. For the common bean, diversity was greatly enhanced at the 10-4 dilution, while for the soybean dilution decreased diversity. Qualitative differences were also observed, as the DNA profiles differed for each treatment in both host plants. Differences obtained can be attributed to dissimilarity in the history of the introduction of both the host plant and the rhizobia (exotic vs. indigenous), to host-plant specificity, rhizobial competitiveness, and population structure, including ease with which some types are released from microcolonies in soil. Therefore, sampling method should be considered both in the interpretation and comparison of the results obtained in different studies, and in the setting of the goals of any study, e.g. selection of competitive strains, or collection of a larger spectrum of rhizobia. Furthermore, effects of sampling should be investigated for each symbiosis.
650 $asoybeans
650 $aRhizobium
650 $aSoja
653 $aDiversidade genética
653 $aRizóbio
700 1 $aKASCHUK, G.
700 1 $aHUNGRIA, M.
773 $tSoil Biology and Biochemistry, Oxford$gv. 38, p. 1298-1307, 2006.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Registro |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
25/11/2016 |
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Data da última atualização: |
20/02/2017 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
FERNANDES, M. S.; MORAIS, P. B.; NASCIMENTO, R. C. do; GARCIA, L. C.; CAPDEVILLE, G. de; BRASIL, B. dos S. A. F. |
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Afiliação: |
MAIARA SOUSA FERNANDES; PRISCILA BORGES MORAIS; RODRIGO CARVALHO DO NASCIMENTO; LORENA COSTA GARCIA, CNPAE; GUY DE CAPDEVILLE, CNPAE; BRUNO DOS SANTOS ALVES FIGUEIREDO B, CNPAE. |
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Título: |
Efeito da morfologia celular e fase de crescimento algal na eficiência da criopreservação de microalgas. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 3., 2016, Brasília, DF. Anais ... Brasília, DF: Embrapa, 2016. |
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Páginas: |
p. 95-101. |
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Idioma: |
Português |
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Palavras-Chave: |
Arquitetura celular; Avaliação da eficiência de criopreservação; Ciclo de crescimento; Cocóide unicelular; Colonial cenobial; Colonial palmeloide; Microalgas. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/150622/1/III-EnPI-2016-97-103.pdf
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Marc: |
LEADER 00912nam a2200253 a 4500
001 2057181
005 2017-02-20
008 2016 bl uuuu u00u1 u #d
100 1 $aFERNANDES, M. S.
245 $aEfeito da morfologia celular e fase de crescimento algal na eficiência da criopreservação de microalgas.$h[electronic resource]
260 $aIn: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 3., 2016, Brasília, DF. Anais ... Brasília, DF: Embrapa$c2016
300 $ap. 95-101.
653 $aArquitetura celular
653 $aAvaliação da eficiência de criopreservação
653 $aCiclo de crescimento
653 $aCocóide unicelular
653 $aColonial cenobial
653 $aColonial palmeloide
653 $aMicroalgas
700 1 $aMORAIS, P. B.
700 1 $aNASCIMENTO, R. C. do
700 1 $aGARCIA, L. C.
700 1 $aCAPDEVILLE, G. de
700 1 $aBRASIL, B. dos S. A. F.
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Embrapa Agroenergia (CNPAE) |
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